How to Read a Peptide Certificate of Analysis Without Overinterpreting It

Research and education notice: This article is educational and does not provide diagnosis, treatment, dosing, preparation or administration guidance. GenLab Meds products are supplied strictly for controlled laboratory and scientific research and are not intended for human or veterinary use or consumption.

To read a peptide Certificate of Analysis (COA), match the compound name and lot number to the vial label. Then check the test date, issuing laboratory, methods and reported results.

Peptide COA checklist

1. Match the lot. Compare the compound and lot number with the vial label.
2. Identify the report. Record its identifier, test date and issuing laboratory.
3. Check the methods. Identify the test used for each reported result.
4. Read the results. Compare the values and units with the applicable specifications and note any missing tests.

Start with traceability

Check the product name, lot number, report identifier, test date and issuing laboratory. A generic certificate without a matching lot cannot characterize a specific vial.

Separate analytical questions

Mass spectrometry may support identity. Reverse-phase HPLC may describe chromatographic purity. Peptide content, water, counter-ion and residual-solvent measurements address other characteristics. One percentage cannot answer every question.

Frequently asked questions

Does 99% HPLC purity mean 99% peptide by vial weight?

No. Peak-area purity is not automatically net peptide content.

Does a COA prove sterility?

Not unless a relevant, valid sterility result is included.

Continue with Peptide Purity vs Identity vs Content and the supplier checklist.

Research and education notice: This article is educational and does not provide diagnosis, treatment, dosing, preparation or administration guidance. GenLab Meds products are supplied strictly for controlled laboratory and scientific research and are not intended for human or veterinary use or consumption.